A cryo-EM atlas of proteins on nucleosomes
Fourteen structures showing how designed proteins meet packaged DNA, and why the angle of approach matters.
Code
EM-09
Year
2024
Status
Published
Modality
Cryo-electron microscopy
Funding
National Research Foundation
Team
Rana Okafor, Theo Brandt, Ilse Maraval
Metric
2.9 Å
Measured
best resolution
Why structures
Two proteins with the same measured affinity can behave differently once the DNA is packaged. We suspected the difference was geometric, so we looked.
The atlas
We solved fourteen structures between 2.9 and 3.8 Å. Proteins that meet the DNA where it leaves the nucleosome sit cleanly; those that meet the histone surface are blocked.
Open data
Every map, model and processing script is public. Three other groups have used the atlas to redesign their own proteins.
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